Electrophoresis of human blood plasma

Gradient gel electrophoresis of human plasma lipoproteins.

A rapid and simple method for separating and identifying plasma lipoproteins is described. The technique utilizes the “molecular sieving” properties of continuous concave gradient electrophoresis on a slab of polyacrylamide (4 to 26%) gel. Gradient gel electrophoresis is particularly suitable for separating lipoproteins according to their molecular weight. The technique can be used to demonstra...

Two-dimensional electrophoresis of human plasma apolipoproteins.

The two-dimensional electrophoretic method with silver staining we describe better resolves plasma apolipoproteins (apo) than any procedure previously described. It can be used to screen for abnormalities in apoA-I, apoA-II, apoA-IV, apoC-II, apoC-III, apoD, apoE, and apoH. In addition, this is the first presentation of apoD and apoH on two-dimensional gels. This electrophoretic method will per...

Xcvii. Electrophoresis of Human Blood Plasma Electrophoretic Properties of Fibrinogen by Einar Stenhagen

High resolution two-dimensional electrophoresis of human plasma proteins.

The two-dimensional electrophoretic technique of O'Farrell has been adapted to the analysis of human plasma proteins, and 30 polypeptides have been identified in the pattern produced. Genetic variants involving charge (isoelectric point) or size (molecular weight in the presence of sodium dodecyl sulfate) changes should be routinely detectable in at least 20 proteins at once, facilitating studi...

Determination of dehydroepiandrosterone in human blood plasma.

The first method for the determination of dehydroepiandrosterone (DHEA) in blood plasma was published by Migeon (1). To follow variations of DHEA in the blood within a rather short period, it seemed desirable to reduce not only the time necessary for one analysis, but also the volume of plasma needed for this procedure. Furthermore, by using the method of Migeon, our recoveries of added Na DHEA...